Extraction-free COVID-19 Testing in Human Saliva

As countries scale up on COVID-19 testing as a means for early detection and containment, there have been concerns on the stock availability of swabs and RNA extraction kits required to perform existing COVID-19 diagnostic tests. Moreover, the process of collecting nasopharyngeal swab specimen may cause anxiety, pain and discomfort in patients. To address these, Veredus Laboratories offers a safer and simpler approach to test directly from human saliva with the combined use of a collection device for saliva, known as ZeroPrep™ Saliva Collection Kit, together with a detection test, known as VereRT™ ZeroPrep™ COVID-19 PCR Kit.

ZeroPrep™ Saliva Collection Kit is a specimen device intended for the collection of human saliva with a preservation buffer designed to protect and preserve viral RNA suitable for use directly in genetic and molecular diagnostic testing. Saliva is collected until liquid saliva fills the 1 ml line on the Collection Tube before adding the ZeroPrep™ Saliva Buffer to generate a saliva mixture. This saliva mixture can then be used for direct COVID-19 diagnostic testing or stored at room temperature (15oC to 30oC or 59oF to 86oF) for up to five days before discarding it as biohazard waste.

VereRT™ ZeroPrep™ COVID-19 PCR Kit is a real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR)-based In Vitro Diagnostic (IVD) test intended for the qualitative detection of nucleic acid belonging to SARS-CoV-2 on a qPCR thermal cycler. This kit has obtained approval from the Health Sciences Authority (HSA) Singapore for IVD use in Singapore.

This real-time RT-PCR test delivers a test sensitivity of Limit of Detection (LoD) as low as 10 viral RNA copies per test of human saliva specimen, with no cross-reactivity with human genome / other coronaviruses / respiratory viruses. Setting up your real-time RT-PCR is simple with premixed reagents, so you can free up time to make decisions that matter in your work.

1. Spit into the funnel until the saliva liquid (not bubbles) reaches the blue fill line. DO NOT overfill.

2. Pour all of the ZeroPrep™ Saliva Buffer through the funnel into the collection tube.

3. Remove the funnel and screw the cap on tightly to prevent spillage.

4. Shake the tube VIGOROUSLY for at least 30 seconds. Place the tube into the Biohazard Bag, then the Ziplock Bag.